An external file that holds a picture, illustration, etc. The classic rat strains for studying dietinduced obesity are wistar and spraguedawley. Development of a new dietinduced obesity dio model using. Dio in the context of alternative models of obesity. Propensity to highfat dietinduced obesity in rats is associated with. Figure 1 shows body weight and cumulative food intake in the dio and dr rats fed either chow or he diet from weaning to. Highfat dietinduced metabolic syndrome and oxidative stress in obese rats are ameliorated by yogurt supplementation. The most widely used models to induce obesity in rats are a lesion of the. Stern, and jules hirsch rockefeller university, new york, new york 10021 faust, irving m.
Ii in a diet induced obesity state, dio rats show insensitivity to the damphetamine effect on icss, but show equal sensitivity. Dietinduced adipocyte number increase in adult rats. Its impact on body weight, lipid metabolism and insulin. Pdf experimental model to induce obesity in rats researchgate. Treating high fat fed rats with enalapril or menhaden oil enriched diet also improved glucose clearance compared to diet induced obese rats but was not as efficacious as replacing the high fat diet with a normal diet figure 1a, auc. I before the onset of obesity dio rats show an innate decreased sensitivity to the effects of damphetamine on icss threshold and feeding, as compared to dr rats. Pdf propensity to highfat dietinduced obesity in rats. Lewcrl rats fed a 60% kcal hfd become obese, but they lack significant metabolic abnormalities frequently. To assess some of the features of the metabolic syndrome, oral glucose tolerance tests, systolic blood pressure. Wistar and sd rats were assigned for 2 experimental groups for 17 weeks. The same variables were then evaluated after exposing the rats to a highfat diet, after dio rats selectively developed obesity.
Since rats and mice show a similar relationship, they are considered an appropriate model for studying dietary obesity. Meal pattern analysis of dietinduced obesity in susceptible and resistant rats article pdf available in obesity research 117. Highfat diet induced obesity causes an inflammatory microenvironment in the kidneys of aging longevans rats article pdf available in journal of inflammation 161 december 2019 with 47 reads. Highfat dietinduced obesity causes an inflammatory. However, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf diet induced obesity has not been adequately evaluated so far. Chronic central oxytocin infusion was carried out in high fat dietinduced obese rats. Adult male hooded rats which were offered a mixed, high energy diet for 90 days were hyperphagic and became significantly obese compared to chowfed control rats. Highfat diet increases pain behaviors in rats with or. Placing obese rats on a normal diet improved glucose utilization.
Highfat dietinduced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. Longterm characterization of the dietinduced obese and diet. Modeling dietinduced obesity with obesityprone rats. Biometric evidence of dietinduced obesity in lewcrl rats ncbi. Some studies have shown a relationship between obesity and infertility, but until now it remains controversial. Dietinduced obesity in rats is associated with glucose intolerance during pregnancy but not in the nonpregnant state. Mechanisms of the antiobesity effects of oxytocin in dietinduced. Typically mice, rats, dogs, or nonhuman primates are used in these models. In this study, chronic administration of hfd in rats produced hypercholesterolemia 116. We applied a comparative functional genomics approach to evaluate whether diet. The dietinduced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. Biometric evidence of dietinduced obesity in lewcrl rats. Pdf the etiology of obesity is multifactorial and is becoming a problem of. The data showed that hfdinduced obesity in lev rats was accompanied.
Steatosis but not peripheral neuropathy was improved after placing obese or. Obesity is rapidly becoming a worldwide epidemic that affects children and adults. Thus, the aim of the present study was to investigate the effect of highfat dietinduced obesity on male reproductive parameters. Propensity to highfat diet induced obesity in rats is associated with changes in the gut microbiota gut inflammation august 2010 ajp gastrointestinal and liver physiology 2992. Propensity to highfat dietinduced obesity in rats is associated with changes in the gut microbiota and gut. Pdf highfat dietinduced obesity causes an inflammatory. Dietinduced obesity in rats leads to a decrease in sperm. The development of hypertension in a rat model of diet induced obesity is described in the literature. Pdf dietinduced obesity in rats leads to a decrease in sperm. In the past decades, obesity and associated metabolic complications have reached epidemic proportions.
However, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf dietinduced obesity has not been adequately evaluated so far. This obese rat model is an attractive model to study further the physiologic and molecular abnormalities in gdm. Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or type 2 diabetes. In the present study, the methanolic extract of moringa oleifera leaves memol was evaluated for antiobesity activity in rats. Pdf meal pattern analysis of dietinduced obesity in. Development of a new dietinduced obesity dio model using wistar lean rats. Antiobesity and hypolipidemic activity of moringa oleifera. Highfat diet consumption for 8 weeks affected body weight in longevans but not spraguedawley rats. The antiobesity potential of memol was studied against high fat dietinduced obesity hfd in rats. Fasting plasma insulin and glucose levels were initially elevated in the experimental rats, but later in the 90 day period were similar to control levels.
Highfat dietinduced metabolic syndrome and oxidative stress in. This is likely to result from the additive effects of obesity and pregnancy on insulin sensitivity. For the study of these pathologies, a number of animal models have been developed. Rats genetically prone to dietinduced obesity dio and their counterpart dietresistant dr were fed a chow diet and their response to damphetamine on intracranial selfstimulation and food intake were assessed. The dietinduced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or high density diets. It is intended to mimic the most common cause of obesity in humans. We examined whether reversal of high fat diet, stimulating weight loss, compared to two treatments previously shown to have beneficial effects, could improve glucose utilization and peripheral neuropathy in animal models of obesity and type 2 diabetes. In a first experiment, male wistar rats were fed a highfat diet hfd. In this study, we used the paradigm of dietinduced obesity first described by woods et al.
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